Study on the Limit Detection Method for Three Diester Alkaloids in Renshen Zaizao Pills
Objective To establish a limit detection method for three types of diester alkaloids in renshen zaizao pills.Methods The content of diester alkaloids(mesaconitine,hypaconitine,aconitine)in renshen zaizao pills was determined by UPLC-MS/MS,with acetonitrile and 0.1%formic acid solution as mobile phase,gradient elution,electrospray ion source,and multi reaction monitoring mode under positive ion mode.Results The linear relationship of the three types of diester alkaloids was good,and the RSD for precision,stability,and repeatability tests were all less than 5%.The average recovery rate and RSD were 96.14%to 100.38%and 1.59%to 2.86%,respectively.Conclusion The established method can accurately and quickly determine the content of three diester type alkaloids in renshen zaizao pills,with strong specificity and good repeatability.It can provide reference for the comprehensive and effective control of the quality of renshen zaizao pills.
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