Content Determination of Asperosaponin Ⅵ in Yaoxitong Capsules by UPLC-MS/MS
Objective To establish an ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the determination of asperosaponinⅥ in yaoxitong capsules.Methods The liquid phase was performed on a Waters ACQUITY UPLC® BEH C18 column(2.1 mm×100 mm,1.7 μm)with mobile phase consisted of acetonitrile-0.1%formic acid(gradient elution)at the flow rate of 0.3 mL/min.The column temperature was 35℃,and the sample size was 5 μL.The mass spectrometry conditions were electrospray ion source(ESI),positive ion scanning mode,and multi-reaction monitoring mode(MRM).The quantitative ion pair of asperosaponin Ⅵ was m/z 946.4→455.3.Results Asperosaponin Ⅵ showed a good linear relationship(r=0.998 7)in the concentration range of 0.078 7 to 3.932 7 μg/mL.RSDs of precision,repeatability and stability were 2.5%,4.1%and 5.3%,respectively,and the average recovery rate was 95.9%.The content of asperosaponin Ⅵin 16 batches of samples ranged from 18.07 to 659.78 μg/g.Conclusion The method can be used to determine the content of asperosaponin Ⅵ in yaoxitong capsules,which is helpful to improve the quality of the preparation.
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