Study on the Inhibitory Effect of Inokosterone on Osteosarcoma Cells
Objective To investigate the effects of inokosterone on the proliferation and apoptosis of osteosarcoma cells.Methods Osteosarcoma MG63 and HOS cells were treated with the different concentrations of inokosterone(0,1,5,10,20 mg/L),to determine the optimal drug intervention concentration through CCK8,and then the optimal drug concentration was used to intervene in osteosarcoma MG63 and HOS cells as the experimental group,while the control group was not treated with inokosterone.The effects of inokosterone on osteosarcoma cell proliferation and apoptosis were observed through CCK8,plate cloning,and flow cytometry experiments.Results After intervention with different concentrations of the drug at the same time,the proliferation of osteosarcoma cells was changed.When the drug concentration was 10 mg/L,the proliferation ability of osteosarcoma cells significantly decreased(P<0.01).At the same concentration,the proliferation ability of cells was significantly inhibited with the extension of time.After 72 hours,the proliferation of osteosarcoma cell markedly decreased(P<0.01).The subsequent experiments were all intervened with 10 mg/L,and the subsequent experimental indicators were measured.Compared with the control group,the proliferation ability of cells in the experimental group was significantly inhibited,the apoptosis rate of cells in the experimental group significantly increased(P<0.01).Compared with the control group,the ability of osteosarcoma cells to form colonies in the inokosterone group significantly decreased(P<0.01).Conclusion Inokosterone has the effects of inhibiting the proliferation of osteosarcoma cells and promoting cell apoptosis.
NETL
NSTL
万方数据
