Mechanism Study of Matrix Gla Protein Inhibiting the Progression of Cutaneous Squamous Cell Carcinoma
Objective To explore the mechanism by which matrix Gla protein(MGP)inhibits the progression of cutaneous squamous cell carcinoma(cSCC).Methods Immunohistochemistry was used to detect the expression of MGP in 30 pairs of cSCC tissues and normal adjacent tissues.MGP gene expression analysis was performed on two independent SCC datasets(GSE122370 and GSE53462)using the Cancer Genome Atlas database.Two cSCC cell lines(A431 and SCL-1)were used as research objects to construct MGP overexpression stable transgenic cell lines A431 and SCL-1,and MTS assay was used to detect the proliferation ability of cSCC cells.The effects of MGP overexpression and knockdown on apoptosis-related proteins in cSCC cells were verified by Western blot.Results The expression of MGP in cSCC tissue was significantly lower than that in adjacent normal tissue(P＜0.05),which was confirmed by analysis of two online datasets.MGP overexpression inhibited the proliferation of cSCC cells A431 and SCL-1(P＜0.05).After overexpression of MGP,the proportion of apoptosis in cSCC cells A431 and SCL-1 significantly increased(P＜0.05).After upregulating MGP,the expression level of BCL-2 was significantly downregulated in A431 and SCL-1 cell lines,while the expression of Caspase3 and Bax was significantly upregulated.After knocking down MGP,the expression level of BCL-2 was significantly upregulated in A431 and SCL-1 cell lines,while the expression of Caspase3 and Bax was significantly downregulated.Conclusion MGP shows low expression in cSCC tissues,which inhibits cell proliferation and promotes apoptosis;in terms of mechanism,MGP inhibits the proliferation of cSCC cells,promotes the apoptosis of cancer cells,and inhibits the occurrence and development of cSCC by regulating the expression of middle note apoptosis related proteins in cSCC cells.
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维普
