Study on Improvement in Quality Standards of Gukang Capsules
Objective To study the thin layer identification and content determination of gukang capsules,and improve its quality standards.Methods TLC was used with banana root as the reference material and petroleum ether(60-90 ℃)-ethyl acetate(20∶1)as the developing agent.HPLC method was established to determine the content of acacetin-6-C-β-D-glucopyranoside,using Diamonsil C18 column(250 mm ×4.6 mm,5 μm)as the chromatographic column,mobile phaseacetonitrile-0.1％phosphoric acid solution(20∶80),330 nm,30 ℃,1.0 mL/min.Results The established TLC identification method had clear spots and good separation effect.The linear relationship between acacetin-6-C-β-D-glucopyranoside and its concentration was good within the range of 1.03～51.35 μg/mL(r=0.999 7),RSD less than 1.00％.Recovery rate was 104.68％,RSD 0.74％.Conclusion The method is simple and feasible and can effectively control the quality of gukang capsules.
万方数据
维普
