Objective To study the thin layer identification and content determination of gukang capsules,and improve its quality standards.Methods TLC was used with banana root as the reference material and petroleum ether(60-90 ℃)-ethyl acetate(20∶1)as the developing agent.HPLC method was established to determine the content of acacetin-6-C-β-D-glucopyranoside,using Diamonsil C18 column(250 mm ×4.6 mm,5 μm)as the chromatographic column,mobile phaseacetonitrile-0.1％phosphoric acid solution(20∶80),330 nm,30 ℃,1.0 mL/min.Results The established TLC identification method had clear spots and good separation effect.The linear relationship between acacetin-6-C-β-D-glucopyranoside and its concentration was good within the range of 1.03～51.35 μg/mL(r=0.999 7),RSD less than 1.00％.Recovery rate was 104.68％,RSD 0.74％.Conclusion The method is simple and feasible and can effectively control the quality of gukang capsules.
维普
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