Objective To improve the quality control standard of wulin pills. Methods Based on the original standard,the TLC identification methods of chlorogenic acid,gardenoside,paeoniflorin,angelica and ligusticum wallichii were added,and the HPLC content determination method of gardenoside and paeoniflorin was established:Anpel Athena C18 (250 mm×4.6 mm,5 μm) was used to chromatgraphic separation with 0.1％ phosphoric acid-acetonitrile (89∶11) as the mobile phase at a flow velocity of 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was set at 240 nm. Results The methodological validation showed that the established TLC methods were proved to be no negative interference,clear spots and good durability. A good linear relationship between the peak area and the content of gardenoside and paeoniflorin was at 0.0454～0.8173 μg (r=0.9997) and 0.0427～0.5125 μg (r=0.9996),respectively. The average recovery rates were 96.0％ and 103.2％,and their RSDs were 1.6％ and 0.9％,respectively. Conclusion The established methods in this study have strong specificity,good reproducibility and durability,which could be used to control the quality of wulin pills.
万方数据
维普
