Study on the Extraction Process of Polysaccharide from Prunellae Spica by Enzymatic Hydrolysis
Objective The purpose of this study was to explore the process of enzymatic extraction of water-soluble polysaccharides from prunellae spica. Methods With the extraction rate of polysaccharide as the index,the effects of enzyme type,extraction temperature,extraction pH,enzyme addition amount and reaction time were investigated by single factor experiment. The extraction conditions were optimized by response surface method,and the antioxidant activity of polysaccharides obtained enzymolysis and hydrolysis was compared. Results The optimum extraction conditions were as follows:enzymolysis temperature 52 ℃,pH 6.1,cellulase dosage 0.22 g,enzymolysis time 107 min. Under these conditions,the extraction rate of prunella polysaccharide was (7.59±1.36)％. The results showed that the DPPH scavenging ability of prunella polysaccharides enzymolysis and hydrolysis was different,and the half inhibitory concentration (IC50) was 0.538 mg/mL and 0.062 mg/mL,respectively. Conclusion This study improved the extraction rate of prunella polysaccharide and provided a good theoretical basis for its clinical application.
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