首页|Anti-proliferation and radiosensitization effects of chitooligosaccharides on human lung cancer line HepG2
Anti-proliferation and radiosensitization effects of chitooligosaccharides on human lung cancer line HepG2
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Objective:To observe the anti-proliferation and radiosensitization effect of chitooligosaccharides (COS) on human lung cancer cell line HepG2.Methds:CCK-8 assay was employed to obtain the inhibition ratio of COS on HepG2 cells at 24 h after treatment.The clonogenic assay was used to analyze the cell viability of RAY group and RAY+COS group with X-ray of 0,1,2,4,6 and 8 Gy,and the cell survival curve was used to analyze the sensitization ratio of COS.Flow cytometry was employed to detect cell cycle and apoptosis rate in control group,RAY group and RAY+COS group after 24 h treatment.Results:COS inhibited the proliferation of HepG2 cells,and the inhibition rate positively correlated with the concentration of COS.The cell viability decreased with increasing exposure dose in RAY group and RAY+COS group.The cell viabilities of RAY+COS group were lower than those of RAY group at the dose of 4,6 and 8 Gy (P＜0.05),and the sensitization ratio of COS was 1.19.There were higher percentage at G2/M phase and apoptosis rate,and lower percentage at S phase in RAY+COS group versus the other two groups (P＜0.01).Conclusions:COS can inhibit the proliferation of HepG2 cells,and enhance the radiosensitization of HepG2 cells,induce apoptosis and G2/M phase arrest.
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NSTL
万方数据
