Identification and Expression Analysis of PYL Gene Family in Eriobotrya japonica
Abscisic acid(ABA)is one of the important hormones regulating plant growth and development,and abiotic stress.As an ABA receptor,PYL is located at the top of its signaling pathway and plays a core regulatory role.However,this gene family has not been identified and analyzed in detail in loquat.In this study,12 PYL genes were identified in loquat based on bioinformatics analysis.The molecular weight of the coding PYL protein ranged from 10.47 to 29.12 kDa,and most of them were localized in the cytoplasm.PYL family genes were distributed on 8 chromosomes.Phylogenetic tree analysis showed that PYL gene family could be divided into three subfamilies and EjPYL1 was closely related to AtPYL13.PYL proteins in loquat were consisted of motif 1-6,and motif3 was the common domain.Genes of PYL family were collinearity in species loquat.There were 2 pairs of collinearity between loquat and Arabidopsis thaliana.Cis-components in promoter of PYL genes were related to low temperature,drought,light and hormone response.RNA-seq of loquat young fruits indicated that EjPYL6 and EjPYL8 were both up-regulated by low temperature in fruit pulp and seeds.This study can provide theoretical basis for further understanding ABA signal transduction pathway and breeding new varieties of loquat with frost resistance.
万方数据
维普
