The quadratic saturated D-optimal regression design experiment was used to study the relationship between the curing environment and the comprehensive score of chemical components of Nicotiana tabacum leaves in the yellowing stage of Yunyan 87.The results show that the influence of different factors on the comprehensive score of chemical components is temperature effect>temperature,dry-wet difference interaction effect>dry-wet temperature difference effect;Under the condition of constant wet-dry difference,the temperature effect equation Y1=83.59-0.89X1+6.35X12,that is,with the increase of temperature,the comprehensive score of chemical components first decreases and then increases.When the temperature X1=39.21℃,the comprehensive score Ymin=83.56;Under the condition of constant temperature,the equation of dry-wet difference effect Y2=83.59+3.32X2-4.63X22,that is,with the increase of dry-wet difference,the comprehensive score of chemical components first increases and then decreases.When the dry-wet difference X2=3.72℃,Ymax=84.19;Under the interactive effect of temperature and dry-wet difference,the comprehensive score of chemical components in the yellowing stage of upper tobacco leaves was higher when the temperature was 36-36.75℃(dry-wet difference was 2-4℃)and 41.3-42℃(dry-wet difference was 3.5-5℃).According to the comprehensive score of chemical composition of tobacco leaves,dry bulb temperature of 42℃,wet bulb temperature of 37.0℃and relative humidity of 69%are the best combination of temperature and humidity for the quality of upper Nicotiana tabacum leaves.