Induction of Callus and Adventitious Buds of Garcinia xanthochymus Seeds
Garcinia xanthochymus seeds exhibit the characteristic of totipotent germination,yet therein physiological basis remains unclear.To investigate the regenerative growth characteristics,explants from mature and immature seeds of G.xanthochymus were utilized to examine the impacts of growth regulators(NAA,TDZ,IAA,6-BA,and 2,4-D)on callus induction and adventitious bud formation,with MS as basic media.The results indicated that:(1)Callus formation primarily depended on the maturity of the seeds.Explants derived from mature seeds exhibited induction rates exceeding 80%even without exogenous growth regulators,while explants sourced from immature seeds only responded to TDZ(0.25-3 mg·L-1)and 6-BA(1.5-2 mg·L-1)with a callus induction rate below 40%.(2)Only 6-BA induced adventitious buds steadily in this study,with the optimal approach being the cultivation of explants from mature seeds on MS medium supplemented with 5.5 mg·L-1 6-BA,resulting in a 73%incidence rate and an average of 5.07 adventitious shoots each explant.These findings further validated the strong regenerative growth potential of G.xanthochymus seeds,and the investigated plantlet regeneration protocol provided a reliable method for asexual propagation in G.xanthochymus.
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