茅莓鉴别与空心莓组ITS序列分析
Identification of Rubus Parvifolius L.and ITS Sequence Analysis of Sect.Idaeobatus
卢昌华 1石锦秀 2唐耿秋 1陈章 1张鑫1
作者信息
- 1. 潮州卫生健康职业学院药学与检验学院,广东潮州 521000
- 2. 广州中医药大学金沙洲医院,广东 广州 510006
- 折叠
摘要
目的:通过对茅莓(Rubus parvifolius L.)的鉴别,为茅莓的开发利用,质量标准制定提供理论依据.方法:采用性状鉴定、显微鉴定、薄层色谱鉴定及分子鉴定进行研究,对空心组植物ITS序列进行分析.结果:茅莓根和茎均有草酸钙簇晶、草酸钙方晶和具缘纹孔导管;叶中有两种非腺毛,一种棒状腺毛.薄层色谱可以有效鉴别茅莓.内部转录间隔区(ITS)序列作为DNA条形码,可以将52种空心莓组植物区分,得到与传统分类不同的分组,遗传距离推测绒毛叶亚组和柔毛叶亚组可能为其他亚组的祖先.结论:该结果可为茅莓及其同组植物的进一步开发利用提供参考依据.
Abstract
Objective:To provide a theoretical basis for the development and utilization of Rubus parvifolius L.and the development of quality standards by identification.Methods:The methods of character identification,microscopic identi-fication,TLC identification and molecular identification were used.The ITS sequence of Sect.Idaeobatus'plants was an-alyzed.Results:There are calcium oxalate clusters,calcium oxalate square crystals and marginal pitted conduits in both roots and stems.There are two types of non-glandular hair and one clav-shaped glandular hair in the leaves.TLC can be used to identify the Rubus parvifolius L.berry effectively.The internal transcriptional spacer(ITS)sequence can be used as a DNA barcode to distinguish 52 species of Sect.Idaeobatus,which is different from the traditional classifica-tion.The genetic distance suggested that the subsect.stimulantes and the subsect.pungentes might be the ancestors of other subgroups.Conclusion:The results can provide reference for the further development and utilization of the Rubus parvifolius L.
关键词
茅莓/性状鉴别/显微鉴别/薄层色谱鉴别/空心莓组内部转录间隔区(ITS)序列Key words
Rubus Parvifolius L./Character Identification/Microscopic Identification/Thin-Layer Chromatography(TLC)Identification/Internal Transcriptional Spacer(ITS)Sequence of Sect.Idaeobatus引用本文复制引用
出版年
2024
NETL
NSTL
万方数据