Study on the Effect of Combination Acupoint Administration of White Mustard Seed and Asarum in Vitro Transdermal Penetration
Objective:To explore the effects of the compatibility of white mustard seed and Asarum,as well as the ad-ministration at ST36 acupoint and non-acupoint,on the in vitro transdermal absorption of the main component of white mustard seed-sinapine thiocyanate and the main element of as arum-asarinin.Methods:Part of the skin of rheumatoid arthritis rabbits were selected and divided into:the acupoint group(ST36)and the non-acupoint group(2 cm away from ST36).At the same time,the white mustard seed single ingredient group,the asarum single ingredient group,and the drug pair compatibility group were divided into dosage groups,for a total of six groups.Franz transdermal diffusion cell and UPLC were used to investigate the cumulative release of sinapine thiocyanate in white mustard seed and asari-nin in asarum,and the cumulative release rate was calculated.A 24 hour Q-t curve was plotted.Results:The Waters UPLC method was used to construct a simultaneous detection method for sinapine thiocyanate and asarinin content.The ST36 acupoint had a promoting effect on the permeability of white mustard seed and asarum single herbs,and its cumulative permeability was higher than that of the non-acupoint group(P＜0.05).Combinations of white mustard seed and asarum,have a certain promoting effect on the permeation of sinapine thiocyanate at both non-acupoints and acupoints.White mustard seed has a certain promoting effect on the permeation of asarinin at acupoints,but it is less obvious at non-acupoints.Results:By comparing the cumulative permeability of sinapine thiocyanate in white mustard seeds after acupoint compatibility and before non-acupoint compatibility,it was found that acupoint administration has a mutual promotion effect with the transdermal promotion of asarum.Comparing the cumulative permeability of asarinin in asarum reveals a mutual promotion effect.White mustard seeds and asarum have a mutual promotion effect,and the drug pair compatibility and acupoint effect have a synergistic promotion effect.The mechanism of action needs further research.
Acupoint TherapyRheumatoid ArthritisWhite Mustard Seed and Asarum Drug PairingVitro Transder-mal Penetration
万方数据
维普
