Exploring the Potential Mechanism of Mahuang Fuzi Xixin Decoction in Treating Elderly Bronchial Asthma Based on Network Pharmacology and Molecular Docking Technology
Objective:Using network pharmacology and molecular docking techniques,this study explores the core active components,key target genes,and potential mechanisms of action of Mahuang Fuzi Xixin Decoction in the treatment of elderly bronchial asthma.Methods:By retrieving and filtering through databases such as TCMSP,Swiss Targets Pre-diction,and UniProt,the active components and related targets of Mahuang Fuzi Xixin Decoction were identified.Using GeneCards,OMIM,and PharmGKB databases,relevant targets for elderly bronchial asthma were searched,and the intersection targets of the drug's active components with the disease were filtered out.A PPI network map was created using STRING and Cytoscape software.The intersection targets of the disease and the herbal active components were imported into the DAVID database for GO and KEGG enrichment analysis to further explore their potential mecha-nisms of action.Using AutoDock Vina software for molecular docking to verify the binding capacity and stability between the core targets and key active components.Results:The study identified 52 active components and 466 target points of these components in Mahuang Fuzi Xixin Decoction,along with 1399 related targets for the disease,narrowing down to 217 intersection targets between the drug and the disease.Through analysis,the core active components of Mahuang Fuzi Xixin Decoction in treating elderly bronchial asthma were identified as quercetin,luteolin,and sinapyl alcohol.The core targets include TNF,IL-6,and AKT1.KEGG analyses mainly involved the TNF and the PI3K/Akt signaling pathway.Molecular docking results showed good binding activity between the core targets and the core active components.Conclusion:Based on network pharmacology and molecular docking,it was concluded that the key active ingredients of Mahuang Fuzi Xixin Decoction combined with the targets of tumor necrosis factor and interleukin 6 and acted on elderly bronchial asthma through the signaling pathways such as tumor necrosis factor and PI3K/Akt,which provided a relevant rationale for the clinical treatment and the experimental data for further research.
Mahuang Fuzi Xixin DecoctionElderly Bronchial AsthmaNetwork PharmacologyMolecular DockingTar-getsMechanism of Action
万方数据
维普
