Mechanism of Rotigotine Long Chain Ester for Improving Olfactory Dysfunction of Parkinsonian Mice
The aim of this experiment is to investigate the mechanism of Rotigotine long chain ester(RLCE)for im-proving olfactory dysfunction of Parkinson's disease(PD).Fifty C57 mice are randomly divided into normal group,model group,RLCE low(11.2 mg·kg-1),medium(22.4 mg·kg-1)and high(44.8 mg·kg-1)dose groups.MPTP-induced PD mouse model is used and RLCE is administered for treatment.The improvement of RLCE on ol-factory dysfunction in mice is observed by Buried Pellet Test and Wood Block Test.Immunohistochemistry(IHC)is used to detect tyrosine hydroxylase(TH)-positive cells in the substantia nigra and olfactory bulb,and the ex-pression levels of α-synuclein(α-syn)and phospho-synuclein(P-α-syn)in olfactory bulb.Western blot is used to detect the expression of α-syn,P-α-syn and inflammation-related factors calcium adaptor protein(IBA1),glial fi-brillary acidic protein(GFAP),and nitric oxide lyase(iNOS)in olfactory bulb.The results show that,RLCE can significantly improve olfactory dysfunction,increase the expression of TH neurons in substantia nigra,decrease the expression of TH neurons in olfactory bulb,and decrease the expression of α-syn,P-α-syn as well as GFAP,iN-OS,and IBA1 in olfactory bulb of mice.The results suggest that RLCE can significantly improve olfactory dysfunc-tion in PD mice,which may be related to the alleviation of neuroinflammatory response,the inhibition of the aggre-gation and phosphorylation of α-syn,and the decrease of the expression of TH neurons in the olfactory bulb.
Parkinson's diseaseolfactory impairmentinflammationRotigotine long chain ester
万方数据
维普
