Constituents Absorbed into Blood and Anti-inflammatory Active Constituents of Shuang Bei Tang Jiang and Isatidis Radix
In order to study the anti-inflammatory substance basis of"Shuang Bei Tang Jiang",UHPLC-Q-Orbitrap HRMS is used to identify the chemical components and constituents can be absorbed into blood of Shuang Bei Tang Jiang and Isatidis Radix.The results of UHPLC-Q-Orbitrap HRMS show that the extract of Isatidis Radix contains 71 main chemical components,46 among of them could enter the blood,while 66 of the above chemical compo-nents are detected in Shuang Bei Tang Jiang,42 of them could enter the blood.Further,the anti-inflammatory ac-tive ingredients and the mechanism of action of its main drug Isatidis Radix are discussed through network pharma-cology,and the anti-inflammatory activity of the main active components are verified by Griess method combined with MTT method.The results of network pharmacologic analysis showed that the key anti-inflammatory components are acacia,isovitexin and eupatorin.5-hydroxymethylfurfural,cytidine,isovitexin and syringic acid significantly inhibite NO release induced by LPS at 100 μmol/L in RAW 264.7 cells.Isovitexin can be detected in serum sam-ples of Shuang Bei Tang Jiang and Isatidis Radix.The results of anti-inflammatory activity experiment show that iso-vitexin has the strongest inhibitory effect on NO production,and maybe the key anti-inflammatory active component of Shuang Bei Tang Jiang and Isatidis Radix.
Shuang Bei Tang JiangIsatidis RadixUHPLC-Q-Orbitrap HRMSconstituents absorbed into bloodnetwork pharmacologyanti-inflammatory effect
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