Sequence Characteristics and Expression Analysis of Tssk2 Gene in Chlamys farreri
In order to study the sequence and expression pattern of testis-specific serine/threonine-protein kinase 2 gene(Tssk2)in Chlamys farreri,the sequence characteristics,tissue expression and gonadal cycle expression of Tssk2 gene are studied by qRT-PCR and bioinformatics analysis.The results are as follows:The CDS(coding se-quences)of Tssk2 is 723 bp long and encodes 240 amino acids.Phylogenetic analysis shows that the evolutionary status of Tssk2 protein in C.farreri is consistent with its biological classification status.Tssk2 possesses the S_TKc conserved domain of the TSSKs,including the serine/threonine protein kinase active region(Ⅵb region)and ATP-binding region,but the ATP-binding region lacks the Ⅰ subdomain.The expression of Tssk2 gene in various tissues and gonadal development cycles of C.farreri show that Tssk2 expression is the highest in the sperms,especially in the sperms at maturity,which shows that this gene may play a key role in mature testes and/or is relates to sperm maturation.
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