Anti-atherosclerosis Mechanisms of Zanthoxylum Bungeanum Maxim.Leaves Based on Network Pharmacology and Molecular Docking
The potential active components and anti-atherosclerosi mechanisms of Z.bungeanum Maxim.leaves are investigated by means of network pharmacology,and verified by molecular docking and cell experiments.The chemical components of Z.bungeanum Maxim.leaves are collected by referring to literatures,and the active com-ponents and related targets are screened by Swiss Target Prediction.Genecards and OMIM databases are used to search the targets related to atherosclerosis.The protein-protein interaction network of Z.bungeanum Maxim.leav-es against atherosclerosis is mapped,and pathway enrichment analysis is conducted with David database.The key components and targets are verified by molecular docking.Finally,the anti-inflammatory effects of key components are verified on lipopolysaccharide stimulated RAW264.7 cells.The results show that 168 active components and 131 potential anti-atherosclerosis targets of Z.bungeanum Maxim.leaves are screened,and the key targets are ob-tained by PPI analysis,including TNF,IL6 and AKT,etc.KEGG enrichment results show that the signaling path-ways,ie AGE-RAGE,VEGF,HIF-1 signaling pathways,involved in the regulation.The results of molecular doc-king of key components and targets show a good bonding.The results of anti-inflammatory experiment show that the key components of Z.bungeanum Maxim.leaves can effectively inhibit the production of NO in lipopolysaccharide stimulated RAW264.7 cells.This study provides theoretical basis for the investigation of anti-atherosclerosis effect of Z.bungeanum Maxim.leaves and beneficial exploration for the development and utilization of Z.bungeanum Maxim.Leaves.
Zanthoxylum bungeanum Maxim.leafatherosclerosisnetwork pharmacologymolecular dockingmechanism of action
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