Process Optimization and Flocculation Mechanism on Biological Flocculation of Scendesmus obliquus by Pleurotus eryngii
To better explore the biological flocculation effect on microalgae,the flocculation of P.eryngii mycelium pellets on S.obliquus cells is investigated.The flocculation process is optimized based on single-factor and orthogo-nal experimental designs,then Zeta potentiometry,fluorometric analysis and fourier infrared spectroscopy are se-lected to disclose the mechanisms of fungal bioflocculation on microalgae from the perspectives of electrostatic inter-action,extracellular polymers.The experimental results show that the optimum flocculation process are:pH=3,flocculation temperature 26℃,rotational speed 180 r/min,and initial microalgae absorbance A0=0.6.The floc-culation rate reaches 85.67%under the above conditions,and the impact sequence is initial microalgae absorbance>rotational speed=temperature>time.S.obliquus is flocculated by P.eryngii mycelium pellets mainly through chemisorption,and other factors including electrostatic interaction between the fungal and microalgal cells,extra-cellular polymers such as proteins and humic acids,functional groups of cell surface also perform important func-tion.This study will provide important references for microalgae flocculation by fungi in a green safe way and im-prove the harvesting rate of microalgae.
万方数据
维普
