Preparation and Evaluation of Compound Anti-hepatocellular Carcinoma Nanomicelles Formulated with Licorice-Epimedium Active Ingredients
Based on the high invasiveness of advanced hepatocellular carcinoma(HCC)and its inherent resistance to conventional chemotherapy,an anti-hepatocellular carcinoma compound nanomicelle was designed and developed to co-deliver the active components of traditional Chinese medicine,icarisideⅡ(ICAⅡ)and glycyrrhetinic acid(GA).Formulation and process optimization were conducted through single-factor and orthogonal experimental de-signs,resulting in the preparation of complex micelles with mPEG-PCL-Phe(Boc)as the carrier material,an ICAⅡ-to-GA mass ratio of 4∶1,drug-accompanying mass ratio of 1∶9,and hydration volume of 4 mL at a water bath temperature of 45℃.The prepared micelles exhibited a typical core-shell structure,with a particle size of(22.30±0.19)nm,PDI of 0.29±0.01,and Zeta potential of(-0.94±0.67)mV,indicating good colloidal stability and biocompatibility.Under simulated physiological conditions(pH 7.4),they can release drugs slowly for up to 10 days,following first-order kinetics.Compared with single drug loaded micelles,the compound micelles signifi-cantly enhanced the in vitro proliferation inhibition activity against human liver cancer cells,demonstrating the anti-hepatocarcinoma potential of the licorice-epimedium active ingredient compound compatibility delivery system.
hepatocellular carcinomaChinese medicine active ingredienticarisideⅡglycyrrhetinic acidcom-pound nanomicelle
万方数据
维普
