The callus induction and proliferation system of quinoa Faro is established through tissue culture assisted cytological observation.The results show that the cotyledon,hypocotyl and seed embryo as explants can induce cal-lus,but there are significant differences in the optimal medium formula:cotyledon explants are MS+0.5mg/L 2,4-D+0.5mg/L 6-BA,cotyl explants are MS+0.5mg/L 2,4-D+0.05mg/L 6-BA,and seed embryo explants are MS+0.2mg/L 2,4-D+0.5mg/L ZT.Callus proliferation mediam is optimized for MS+0.1mg/L 2,4-D+0.2mg/L 6-BA.Increasing sucrose concentration is beneficial to the transformation of callus cells into embryogenic cells.
维普
万方数据