Establishment of Callus Induction and Proliferation System and Histological Observation of Chenopodium Quinoa Willd
The callus induction and proliferation system of quinoa Faro is established through tissue culture assisted cytological observation.The results show that the cotyledon,hypocotyl and seed embryo as explants can induce cal-lus,but there are significant differences in the optimal medium formula:cotyledon explants are MS+0.5mg/L 2,4-D+0.5mg/L 6-BA,cotyl explants are MS+0.5mg/L 2,4-D+0.05mg/L 6-BA,and seed embryo explants are MS+0.2mg/L 2,4-D+0.5mg/L ZT.Callus proliferation mediam is optimized for MS+0.1mg/L 2,4-D+0.2mg/L 6-BA.Increasing sucrose concentration is beneficial to the transformation of callus cells into embryogenic cells.
万方数据
维普
