首页|Adding chitosan nanoparticles of green tea extract in diluent and thawing temperatures ameliorate the post-thawed quality of Boer buck semen

Adding chitosan nanoparticles of green tea extract in diluent and thawing temperatures ameliorate the post-thawed quality of Boer buck semen

扫码查看
原文链接
  • NETL
  • NSTL
  • 维普
  • 万方数据
Objective:To improve the quality of post-thawing Boer buck semen for artificial insemination by adding green tea extract chitosan nanoparticles to skimmed egg yolk diluent,and the proper thawing temperature.Methods:The ejaculate of Boer buck was added to skimmed egg yolk diluent without(the control group)and with adding 1 μg of chitosan nanoparticles of green tea extract per mL of diluent(the treatment group).Then,the diluted semen was filled in French mini straws containing 60×106 live sperm per straw,frozen in a standard protocol,and stored as frozen semen at-196℃for a week.Six replicates from each group were diluted for 30 s at 37℃or 39℃sterile water to evaluate the semen quality.Results:Post-thawing(at 37℃or 39℃)of live sperm,progressive motility,and plasma membrane integrity were lower compared to those of the pre-freezing stage(P<0.05).Thawing at 37℃resulted in no significant difference in live sperm,progressive motility,and plasma membrane between the control group and the treatment group(P>0.05).The live sperm,progressive motility,and plasma membrane of the treatment group in the pre-freezing stage,and post-thawed at 39℃were higher compared to those of the control group(P<0.05).There was no significant difference in malondialdehyde(MDA)concentration,DNA fragmentation,and catalase concentration of thawing at 37℃compared to those of 39℃in the same group.The MDA concentration and DNA fragmentation in thawing at 37℃and 39℃of the treatment group were significantly lower than those of the control group(P<0.05).However,the catalase concentration in thawing at 37℃and 39℃of the treatment group was not significantly different than the control group(P>0.05).Conclusions:Higher quality post-thawing Boer buck semen is achieved by adding 1 μg/mL of chitosan nanoparticles of green tea extract to the skimmed egg yolk diluent and thawing at 39℃.

Boer buck semenChitosan nanoparticlesSemen diluentHigher post-thawing semen qualityPrevent undernutrition

Suherni Susilowati、Imam Mustofa、Tri Wahyu Suparyogi、Adeyinka Oye Akintunde、Djoko Agus Purwanto、Wurlina Wurlina、Suzanita Utama、Sri Mulyati

展开 >

Division of Veterinary Reproduction,Faculty of Veterinary Medicine,Universitas Airlangga,Kampus C Unair,Mulyorejo,Surabaya 60115,Indonesia

Department of Agriculture and Industrial Technology,Babcock University,Ilishan-Remo,Ogun State 121103,Nigeria

Department of Pharmaceutical Chemistry,Faculty of Pharmacy,Universitas Airlangga,Kampus C Mulyorejo,Surabaya 601155,East Java,Indonesia

Universitas Airlangga,Indonesia

1405/UN3.1.6/PT/2022

2024

10.4103/apjr.apjr_93_23

亚太生殖杂志(英文版)

亚太生殖杂志(英文版)

ISSN：
年,卷(期)：2024.13(1)
  • 42