首页|Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development

Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development

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Objective:To investigate the effects of coenzyme Q10(CoQ10)supplementation on post-vitrification embryo development and gross morphology.Methods:Balb/c mouse embryos were cultured in potassium simplex optimised medium(KSOM)with varying CoQ10 concentrations[0(control),20,40,and 60 μM].The most effective CoQ10 concentration(40 μM)was selected for subsequent post-vitrification morphology study.Embryos were randomly divided into four groups:Group A(non-vitrified without CoQ10),Group B(non-vitrified with CoQ10),Group C(vitrified without CoQ10),and Group D(vitrified with CoQ10),followed by vitrification at the 8-cell stage.Survival rates and development until the blastocyst stage were evaluated through morphological examinations using ASEBIR's system,distinguishing normal and abnormal embryos.Results:Supplementation of 40 μM CoQ10 significantly increased blastocyst formation(95%)compared to the control group(92%),20 μM(62%),and 60 μM(56%)(P<0.001).Following vitrification,Group D exhibited a significant increase in blastocyst formation(92%)compared to Group C(82%)(P<0.05).Morphological assessments indicated superior embryo quality in Group B over Group D during the cleavage stage,morula,and blastocyst(P<0.05).Conclusions:CoQ10 supplementation exhibits promising potential to enhance preimplantation embryo development,increase blastocyst formation rates,and improve embryo quality post-vitrification.This offers a promising approach to mitigate oxidative stress on embryos,potentially improving overall assisted reproductive technology outcomes.

Coenzyme Q10AntioxidantEmbryo developmentVitrificationMorphologyAssisted reproductive technology

Anisa-Annur S、Wan-Hafizah WJ、Nor-Ashikin MNK、Muhammad-Zaki R

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Faculty of Pharmacy and Health Sciences,Royal College of Medicine Perak,Universiti Kuala Lumpur,30450 Ipoh,Perak,Malaysia

Maternofetal and Embryo Research Group,Faculty of Medicine,Universiti Teknologi MARA,Selangor Branch,Sungai Buloh Campus,47000 Sungai Buloh,Selangor,Malaysia

2024

10.4103/apjr.apjr_136_23

亚太生殖杂志(英文版)

亚太生殖杂志(英文版)

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年,卷(期)：2024.13(3)