首页|Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea

Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea

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Brassica juncea is an allopolyploid originating from the interspecific hybridization between Brassica rapa and Brassica nigra, which is of multiple usage as a vegetable, oilseed and condiment worldwide. Both vernalization and non-vernalization under long-day photoperiod can promote floral transition in B. juncea suggesting merged flowering pathways of its ancestors and better environmental adaptability. We identified genome-wide flowering regulatory genes in B. juncea, which include 84 and 79 genes from A and B sub-genomes, respectively. Ka/Ks analysis revealed a purification effect on both photoperiod and vernalization flowering regulation pathways during evolution. Expression profile of those genes during long-day and vernalization treatments suggested BjuACO4, BjuAFT1, BjuBFT4, BjuASOC1 and BjuASOC4 may be the major functional copies of B. juncea flowering regulation. Further functional studies about BjuCOs showed three days delayed flowering time in BjuACO4 or BjuBCO3 silenced plants. Increased transcription of all BjuFLCs in BjuACO4 or BjuBCO3 silenced plants suggested interactions between photoperiod and vernalization pathways governing flowering time. Our findings provided flowering regulating networks in allopolyploid B. juncea.

Brassica junceaAllopolyploidyFlowerVernalizationPhotoperiod

Ning Zhao、Chang Liu、Yiqing Meng、Zhongyuan Hu、Mingfang Zhang、Jinghua Yang

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Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China

Key laboratory of Horticultural Plant Growth, Development&Quality Improvement, Ministry of Agriculture, Hangzhou 310058, China

This work was supported by the grant from the National Natural Science Foundation of China

31872095

2019

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN:
年,卷(期):2019.5(3)
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