首页|Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L.ssp.pekinensis)

Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L.ssp.pekinensis)

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Chinese cabbage,belonging to Brassica rapa species,is an important vegetable in Eastern Asia.It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is generally low.The lack of an efficient and stable genetic transformation system for Chinese cabbage has largely limited related gene functional studies.In this study,we firstly developed a regeneration system for Chinese cabbage by optimizing numerous factors,with 93.50％ regeneration rate on average.Based on this,a simple and efficient Agrobacteriummediated genetic transformation method was established,without pre-culture procedure and concentration adjustment of hormone and AgNO3 in co-cultivation and selection media.Using this system,transformants could be obtained within 3.5-4.0 months.Average transformation frequency is up to 10.83％.The establishment of this simple and efficient genetic transformation method paved the way for further gene editing and functional studies in Chinese cabbage.

Chinese cabbageAgrobacterium tumefaciensgenetic transformation

Xiaonan Li、Haiyan Li、Yuzhu Zhao、Peixuan Zong、Zongxiang Zhan、Zhongyun Piao

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Department of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, China

work was supported by the National key research and Development ProgramNational Natural Science Foundation of ChinaNational Natural Science Foundation of Chinaand China Postdoctoral Science Foundationand China Postdoctoral Science Foundation

2017YFD010180231772326317019302016M6013452019T120219

2021

园艺学报(英文版)

园艺学报(英文版)

SCI
ISSN：
年,卷(期)：2021.7(2)
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