首页|ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis

ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration.However,the regulatory network of C.humilis in anthocyanin biosynthesis is still unclear.In this study,the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species.Yeast one-hybrid,dual-luciferase assays,and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C.humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS(MYB-binding elements).ChMYB1 interacted with ChbHLH42 and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT.In addition,abscisic acid(ABA)treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C.humilis fruit.Interestingly,ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42.Furthermore,ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42.Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C.humilis fruit,deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops.

Cerasus humilisAnthocyaninChMYB1FruitABA

Xiaolong Ji、Zhe Li、Mingyu Zhang、Shaoyu Lang、Xingshun Song

展开 >

State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,Heilongjiang 150040,China

Department of Genetics,College of Life Science,Northeast Forestry University,Harbin,Heilongjiang 150040,China

National Natural Science Foundation of ChinaInnovation Project of State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University)Natural Science Foundation of Heilongjiang ProvinceFundamental Research Funds for the Central Universities

321717372021A04LH2021C0072572021DX07

2024

10.1016/j.hpj.2023.05.015

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(1)
  • 56