首页|Overexpression of auxin/indole-3-acetic acid gene MdIAA24 enhances Glomerella leaf spot resistance in apple(Malus domestica)

Overexpression of auxin/indole-3-acetic acid gene MdIAA24 enhances Glomerella leaf spot resistance in apple(Malus domestica)

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Auxin is throughout the entire life process of plants and is involved in the crosstalk with other hormones,yet its role in apple disease resistance remains unclear.In this study,we investigated the function of auxin/indole-3-acetic acid(IAA)gene MdIAA24 overexpression in enhancing apple resistance to Glomerella leaf spot(GLS)caused by Colletotrichum fructicola(Cf).Analysis revealed that,upon Cf infection,35S::MdIAA24 plants exhibited enhanced superoxide dismutase(SOD)and peroxidase(POD)activity,as well as a greater amount of glutathione(reduced form)and ascorbic acid accumulation,resulting in less H2O2 and superoxide anion(O2·-)in apple leaves.Furthermore,35S::MdIAA24 plants produced more protocatechuic acid,proanthocyanidins B1,proanthocyanidins B2 and chlorogenic acid when infected with Cf.Following Cf infection,35S::MdIAA24 plants presented lower levels of IAA and jasmonic acid(JA),but higher levels of salicylic acid(SA),along with the expression of related genes.The overexpression of MdIAA24 was observed to enhance the activity of chitinase and β-1,3-glucanase in Cf-infected leaves.The results indicated the ability of MdIAA24 to regulate the crosstalk between IAA,JA and SA,and to improve reactive oxygen species(ROS)scavenging and defense-related enzymes activity.This jointly contributed to GLS resistance in apple.

AppleMdIAA24Glomerella leaf spot(GLS)Antioxidant capacityAuxinSalicylic acidJasmonic acid

Qian Wang、Dong Huang、Wenyan Tu、Fengwang Ma、Changhai Liu

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State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China

National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaSpecial Funds for Major Scientific and Technological Innovation from Shaanxi ProvinceEarmarked Fund for China Agriculture Research SystemChina Postdoctoral Science FoundationChina Postdoctoral Science Foundation

2018YFD1000307321725292020zdzx03-01-01CARS-272017M6106572018T111108

2024

10.1016/j.hpj.2022.12.011

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(1)
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