首页|A 1-bp deletion in the MC04g1399 is highly associated with failure to produce fruit wart in bitter gourd

A 1-bp deletion in the MC04g1399 is highly associated with failure to produce fruit wart in bitter gourd

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Fruit wart is an important appearance trait influencing consumer preferences of bitter gourd(Momordica charantia L.).The molecular genetic mechanisms underlying fruit wart formation in bitter gourd are largely unknown.In this study,genetic analysis based on four generations showed that fruit wart formation in bitter gourd was controlled by a single dominant locus named as Fwa.The Fwa locus was initially mapped into a 4.82 Mb region on pseudochromosome 4 by BSA-seq analysis and subsequently narrowed down to a 286.30 kb region by linkage analysis.A large F2 population consisting of 2 360 individuals was used to screen recombinants,and the Fwa locus was finally fine mapped into a 22.70 kb region harboring four protein-coding genes through recombination analysis.MC04g1399,encoding an epidermal patterning factor 2-like pro-tein,was proposed as the best candidate gene for Fwa via sequence variation and expression analysis.In addition,a 1-bp insertion and deletion(InDel)variation within MC04g1399 was converted to a cleaved amplified polymorphic sequence(CAPS)marker that could precisely distinguish between the warty and non-warty types with an accuracy rate of 100％among a wide panel of 126 bitter gourd germplasm resources.Our results not only provide a scientific basis for deciphering the molecular mechanisms underlying fruit wart formation but also provide a powerful tool for efficient genetic improvement of fruit wart via marker-assisted selection.

Bitter gourdFruit wartBulk segregant analysisFine-mappingCandidate gene

Jia Liu、Junjie Cui、Jichi Dong、Jian Zhong、Chunfeng Zhong、Fanchong Yuan、Wendong Guan、Fang Hu、Jiaowen Cheng、Kailin Hu

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College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642,China

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China),Ministry of Agriculture and Rural Affairs,Guangzhou,Guangdong 510642,China

Guangdong Vegetables Engineering Research Center,Guangzhou,Guangdong 510642,China

Department of Horticulture,Foshan University,Foshan,Guangdong 528225,China

Henry Fok School of Biology and Agricultural,Shaoguan University,Shaoguan,Guangdong 512023,China

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Science and Technology Planning Project of Guangdong ProvinceScience and Technology Planning Project of Guangdong ProvinceSeed Industry Revitalization Project of Special Funds for Provincial Rural Revitalization StrategyGuangzhou Science and Technology Plan ProjectsGuangzhou Science and Technology Plan ProjectsGuangzhou Science and Technology Plan ProjectsGuangzhou Basic and Applied Basic Research Project

2022 B02021600152019A0505200022022-NPY-00-027202002020086202102020800202206010170SL2023A04J01673

2024

10.1016/j.hpj.2022.12.017

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(1)
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