Styrax japonicus functional genomics:an efficient virus induced gene silencing(VIGS)system

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外文摘要：VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in plants.Since there is no report for available VIGS system in Styrax japonicus,the application of a VIGS approach that results in a gene knockdown to study gene function is limited.In this study,we compared the characteristics that could affect the viability of VIGS in S.japonicus,including the acetosyringone(AS)concentration,the Agrobacterium's optical density and the inoculation method.The stable reference genes of S.japonicus were selected to validate the gene's knockdown by quantitative PCR.As a result,we successfully constructed 2 VIGS systems based on TRV virus:vacuum with AS concentration of 200 μmol-L-1 and OD600 of 0.5,and friction-osmosis with AS concentration of 200 µmol·L-1 and OD600 of 1.0,which silencing efficiency was 83.33％and 74.19％,respectively.The successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in S.japonicus to identify unknown gene functions.

外文关键词：

Styrax japonicusVIGSReference gene

作者：

Gangyu Sun、Yiqian Ju、Cuiping Zhang、Lulu Li、Xinqiang Jiang、Xiaoman Xie、Yizeng Lu、Kuiling Wang、Wei Li

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作者单位：

College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao,Shandong 266109,China

Shandong Provincial Center of Forest and Grass Germplasm Resources,Jinan,Shandong 250102,China

基金：

Subject of Key R & D Plan of Shandong Province(Major Scientific and Technological Innovation Project)Science& Technology SpecificProjects in Agricultural Hightech Industrial Demonstration Area of the Yellow River Delta

项目编号：

2021LZGC02303022SZX16

出版年：

2024

DOI：

10.1016/j.hpj.2023.05.003

园艺学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.10(1)

参考文献量34