首页|AcWRKY28 mediated activation of AcCPK genes confers salt tolerance in pineapple(Ananas comosus)

AcWRKY28 mediated activation of AcCPK genes confers salt tolerance in pineapple(Ananas comosus)

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Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers.In plants,multiple regulatory genes,such as the WRKY transcription factor(TF)family,modulate the expression of defense genes.However,the role of the pineapple WRKY genes is poorly understood.Here,we studied the pineapple WRKY gene,AcWRKY28,by generating AcWRKY28 over-expressing transgenic pineapple plants.Overexpression of AcWRKY28 enhanced the salt stress resistance in transgenic pineapple lines.Comparative transcriptome analysis of transgenic and wild-type pineapple plants showed that"plant-pathogen interaction"pathway genes,including 9 calcium-dependent protein kinases(CPKs),were up-regulated in AcWRKY28 over-expressing plants.Furthermore,chromatin immunoprecip-itation and yeast one-hybrid assays revealed AcCPK12,AcCPK3,AcCPK8,AcCPK1,and AcCPK15 as direct targets of AcWRKY28.Consistently,the study of AcCPK12 over-expressing Arabidopsis lines showed that AcCPK12 enhances salt,drought,and disease resistance.This study shows that AcWRKY28 plays a crucial role in promoting salt stress resistance by activating the expression of AcCPK genes.

PineappleAcWRKYAcCPKTranscription factorSalt stressChIP

Qiao Zhou、Samaranayaka Vidana Gamage Nirosha Priyadarshani、Rongjuan Qin、Han Cheng、Tiantian Luo、Myat Hnin Wai、Mohammad Aqa Mohammadi、Yanhui Liu、Chang Liu、Hanyang Cai、Xiaomei Wang、Yeqiang Liu、Yuan Qin、Lulu Wang

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Guangxi Key Lab of Sugarcane Biology,State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,College of Life Science,College of Plant Protection,College of Agriculture,College of Horticulture,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,Center for Genomics and Biotechnology,Fujian Agriculture and Forestry

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,College of Life Science,College of Plant Protection,College of Agriculture,College of Horticulture,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,Center for Genomics and Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China

National Education Commission,BMICH,Colombo,00700,Sri Lanka

Fishery Multiplication Management Station of Lijiang River Water Supply Hub Project,Guilin,Guangxi 541001,China

Horticulture Research Institute,Guangxi Academy of Agricultural Sciences,Nanning Investigation Station of South Subtropical Fruit Trees,Ministry of Agriculture,Nanning,Guangxi 530007,China

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广西自然科学基金中国博士后科学基金Science and Technology Major Project of Guangxi Gui Keproject of Guangxi featured fruit innovation team on pineapple breeding and cultivation post under national modern agricultu

2022GXNSFBA0355232022MD713731AA22067096nycytxgxcxtd-17-05

2024

10.1016/j.hpj.2023.05.002

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(2)
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