首页|Improved observation of colonized roots reveals the regulation of arbuscule development and senescence by drought stress in the arbuscular mycorrhizae of citrus
Improved observation of colonized roots reveals the regulation of arbuscule development and senescence by drought stress in the arbuscular mycorrhizae of citrus
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Citrus is the typical mycorrhizal fruit tree species establishing symbiosis with arbuscular mycorrhizal(AM)fungi.However,arbuscule development and senescence in colonized citrus roots,especially in response to drought stress,remain unclear,which is mainly due to the difficulty in clearing and staining lignified roots with the conventional method.Here,we improved the observation of colonized roots of citrus plants with the sectioning method,which enabled the clear observation of AM fungal structures.Furthermore,we investigated the effects of one week of drought stress on arbuscule development and senescence with the sectioning method.Microscopy observations indicated that drought stress significantly decreased mycorrhizal colonization(F％and M％)although it did not affect plant growth performance.Fluorescence probes(WGA 488 and/or Nile red)revealed that drought stress inhibited arbuscule development by increasing the percentage of arbuscules at the early stage and decreasing the percentages of arbuscules at the midterm and mature stages.Meanwhile,drought stress accelerated arbuscule senescence,which was characterized by the increased accumulation of neutral lipids.Overall,the sectioning method developed in this study enables the in-depth investigation of arbuscule status,and drought stress can inhibit arbuscule development but accelerate arbuscule senescence in the colonized roots of citrus plants.This study paves the way to elaborately dissecting the arbuscule dynamics in the roots of fruit tree species in response to diverse abiotic stresses.
Arbuscular mycorrhizaeCitrusDrought stressArbuscule developmentArbuscule senescenceFruit tree species
Xilong Yin、Wei Zhang、Zengwei Feng、Guangda Feng、Honghui Zhu、Qing Yao
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College of Horticulture,South China Agricultural University,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China),Ministry of Agriculture and Rural Affairs,Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Guangzhou,Guangdong 510642,China
Key Laboratory of Agricultural Microbiomics and Precision Application,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Key Laboratory of Agricultural Microbiomics,State Key Laboratory of Applied Microbiology Southern China,Guangdong Microbial Culture Collection Center(GDMCC),Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou,Guangdong 510070,China
国家自然科学基金open competition program of top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-open competition program of top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-广东省自然科学基金GDAS Project of Science and Technology Development
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