首页|Chromosome-level assembly of triploid genome of Sichuan pepper(Zanthoxylum armatum)

Chromosome-level assembly of triploid genome of Sichuan pepper(Zanthoxylum armatum)

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As an important spice species in Rutaceae,the Sichuan pepper(Zanthoxylum armatum)can provide pungent and numbing taste,as well as aroma in its mature fruit.Here we assembled a chromosome-level genome of green prickly ash which was widely cultivated in a major production area including Chongqing and Sichuan province,China.We generated 712 Gb(～112 x)PacBio long reads and 511 Gb(～82 x)Hi-C data,and yielded an assembly of 99 pseudochromosomes with total size of 5.32 Gb and contig N50 of 796 kb.The genomic analyses and cytogenetic experiments both indicated that the cultivar'Zhuye Huajiao'was a triploid.We identified a Zanthoxylum-specific whole genome duplication event emerging about 24.8 million years ago(Mya).We also detected a transposition burst event(0.3-0.4 Mya)responsible for the large genome size of Z.armatum.Metabolomic analysis of the Zanthoxylum fruits during development stages revealed profiles of 39 volatile aroma compounds and 528 secondary metabolites,from which six types of sanshools were identified.Based on metabolomic and transcriptomic network analysis,we screened candidate genes encoding long chain acyl-CoA synthetase,fatty acid desaturase,branched-chain amino acid aminotransferase involved in sanshool biosynthesis and three genes encoding terpene synthase during fruit development.The multi-omics data provide insights into the evolution of Zanthoxylum and molecular basis of numbing and aroma flavor of Sichuan pepper.

Zanthoxylum armatumGenomeSanshoolTerpenoid

Lizhi Song、Yue Huang、Hao Zuo、Ning Tang、Zhengguo Li、Wenbiao Jiao、Feng Xu、Qiang Xu、Zexiong Chen

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National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops,Huazhong Agricultural University,Wuhan,Hubei 430070,China

Chongqing Key Laboratory of Economic Plant Biotechnology,Chongqing University of Arts and Sciences,Chongqing 402160,China

School of Life Sciences,Chongqing University,Chongqing 400030,China

College of Informatics,Huazhong Agricultural University,Wuhan,Hubei 430070,China

College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025,China

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Projects for Innovative Research Groups of Chongqing UniversitiesChongqing talent program for Zexiong Chen,Scientific Technological Research Program of Chongqing Municipal Education Commission国家自然科学基金国家重点研发计划

CXQT21028KJZD-K201901303319250342019YFD1001200

2024

10.1016/j.hpj.2022.12.014

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(2)
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