首页|Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato

Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato

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High temperature stress is one of the major environmental factors that affect the growth and development of plants.Although WRKY transcription factors play a critical role in stress responses,there are few studies on the regulation of heat stress by WRKY transcription factors,especially in tomato.Here,we identified a group I WRKY transcription factor,SlWRKY3,involved in thermotolerance in tomato.First,SlWRKY3 was induced and upregulated under heat stress.Accordingly,overexpression of SlWRKY3 led to an increase,whereas knock-out of SlWRKY3 resulted in decreased tolerance to heat stress.Overexpression of SlWRKY3 accumulated less reactive oxygen species(ROS),whereas knock-out of SlWRKY3 accumulated more ROS under heat stress.This indicated that SlWRKY3 positively regulates heat stress in tomato.In addition,SlWRKY3 activated the expression of a range of abiotic stress-responsive genes involved in ROS scavenging,such as a SlGRXS1 gene cluster.Further analysis showed that SlWRKY3 can bind to the promoters of the SlGRXS1 gene cluster and activate their expression.Collectively,these results imply that SlWRKY3 is a positive regulator of thermotolerance through direct binding to the promoters of the SlGRXS1 gene cluster and activating their expression and ROS scavenging.

TomatoWRKY transcription factorSlWRKY3ThermotoleranceSlGRXS1Gene clusterAbiotic stress

Ying Wang、Wenxian Gai、Liangdan Yuan、Lele Shang、Fangman Li、Zhao Gong、Pingfei Ge、Yaru Wang、Jinbao Tao、Xingyu Zhang、Haiqiang Dong、Yuyang Zhang

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National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops,Huazhong Agricultural University,Wuhan,Hubei 430070,China

Hubei Hongshan Laboratory,Wuhan,Hubei 430070,China

National Key Research and Development Plan,ChinaNational Key Research and Development Plan,China国家自然科学基金国家自然科学基金Key Project of Hubei Hongshan LaboratoryWuhan Major Project of Key Technologies in Biological Breeding中央高校基本科研业务费专项International Cooperation Promotion Plan of Shihezi University

2021YFD12002012022YFD120050231972426319911822021hszd00720220213020248522662022YLPY001GJHZ202104

2024

10.1016/j.hpj.2022.12.006

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(2)
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