首页|Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum 'Bangkok Rose'

Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum 'Bangkok Rose'

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Hippeastrum,a highly diverse genus in the Amaryllidaceae family,is a valuable ornamental bulbous flowering plant.Somatic embryogenesis(SE)is an efficient method for mass production of Hippeastrum plantlets.Previous studies have been devoted to the in vitro propagation of Hippeastrum,but the SE and its regulatory networks are rarely reported.In this study,we established a direct SE method of Hippeastrum'Bangkok Rose'using leaf bases as explants.MS supplemented with 1.00 mg·L-1 NAA+1.00 mg·L-1 KT+0.25 mg·L-1 TDZ was the optimal medium for SE.Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation,globular,scutellar and coleoptile stages.During SE,endogenous hormones of IAA,CTK,ABA,and SA were highly accumulated.Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced,while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated,respectively,leading to suppression of auxin signaling.In contrast,cytokine signaling was promoted at the early stage of SE,as biosynthesis,transport,and signaling components were up-regulated.Various stress-related genes were up-regulated at the early or late stages of SE.Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE.Moreover,key SE regulators,including WOXs and SERKs were highly expressed along with SE.Overall,the present study provides insights into the SE regulatory mechanisms of the Hippeastrum.

HippeastrumTissue cultureSomatic embryogenesisGene regulation

Jingjue Zeng、Yi Deng、Shahid Iqbal、Jiarui Zhang、Kunlin Wu、Guohua Ma、Lin Li、Guangyi Dai、Rufang Deng、Lin Fang、Songjun Zeng

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Guangdong Provincial Key Laboratory of Applied Botany,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650,China

University of the Chinese Academy of Sciences,Beijing 100049,China

South China National Botanical Garden,Guangzhou,Guangdong 510650,China

Zhaoqing Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology,Zhaoqing,Guangdong 526000,China

Horticultural Science Department,North Florida Research and Education Center,University of Florida/IFAS,Quincy,FL 32351,USA

Public Laboratory of South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650,China

Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650,China

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Guangdong Basic and Applied Basic Research FoundationDongguan Provincial Rural Revitalization Program(2021)Guangdong Key Technology Research and Development ProgramGuangdong Modern Agricultural Industry Technology System ProgramSouth China Botanical Garden,the Chinese Academy of Sciences

2023A1515010237202118004000222020B0202200052022B11110400032023KJ121QNXM-02

2024

10.1016/j.hpj.2023.02.013

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(2)
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