首页|Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple

Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Abiotic stress reduces plant yield and quality.WRKY transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drought and osmotic stresses in apple(Malus x domestica Borkh.)remain unclear.Here,we functionally characterized the apple Group Ⅲ WRKY gene MdWRKY115.qRT-PCR analysis showed that MdWRKY115 expression was up-regulated by drought and osmotic stresses.GUS activity analysis revealed that the promoter activity of MdWRKY115 was enhanced under osmotic stress.Subcellular localization and transactivation assays indicated that MdWRKY115 was localized to the nucleus and had a transcriptional activity domain at the N-terminal region.Transgenic analysis revealed that the overexpression of MdWRKY115 in Arabidopsis plants and in apple callus markedly enhanced their tolerance to drought and osmotic stresses.DNA affinity puri-fication sequencing showed that MdWRKY115 binds to the promoter of the stress-related gene MdRD22.This binding was further verified by an electrophoretic mobility shift assay.Collectively,these findings suggest that MdWRKY115 is an important regulator of osmotic and drought stress tolerance in apple.

AppleMdWRKY115Osmotic stressDrought stressFunction analysis

Qinglong Dong、Yi Tian、Xuemei Zhang、Dingyue Duan、He Zhang、Kaiyu Yang、Peng Jia、Haoan Luan、Suping Guo、Guohui Qi、Ke Mao、Fengwang Ma

展开 >

College of Forestry,Hebei Agricultural University,Baoding,Hebei 071001,China

State Key Laboratory of Crop Stress Biology for Arid Areas,Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China

National Engineering Research Center for Agriculture in Northern Mountainous Areas,Agricultural Technology Innovation Center in Mountainous Areas of Hebei Province,Hebei Agricultural University,Baoding,Hebei 071001,China

College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071001,China

展开 >

河北省自然科学基金Hebei Apple Innovation Team of Modern Agricultural Industry Technology System国家自然科学基金

C2022204086HBCT202110021132072524

2024

10.1016/j.hpj.2023.05.005

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(3)
  • 61