首页|Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection
Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection
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Apple leaf spot,caused by the Alternaria alternata apple pathotype(AAAP),is an important fungal disease of apple.To understand the molecular basis of resistance and pathogenesis in apple leaf spot,the transcriptomes of two apple cultivars'Hanfu'(HF)(resistant)and'Golden Delicious'(GD)(susceptible)were analyzed at 0,6,18,24 and 48 h after AAAP inoculation by RNA-Seq.At each time point,a large number of significantly differentially expressed genes(DEGs)were screened between AAAP-inoculated and uninoculated apple leaves.Analysis of the common DEGs at four time points revealed significant differences in the resistance of'HF'and'GD'apple to AAAP infection.RLP,RNL,and JA signal-related genes were upregulated in both cultivars to restrict AAAP development.However,genes encoding CNLs,TNLs,WRKYs,and AP2s were only activated in'HF'as part of the resistance response,of which,some play major roles in the regulation of ET and SA signal trans-duction.Further analysis showed that many DEGs with opposite expression trends in the two hosts may play important regulatory roles in response to AAAP infection.Transient expression of one such gene MdERF110 in'GD'apple leaves improved AAAP resistance.Collectively,this study highlights the reasons for differential resistance to AAAP infection between'HF'and'GD'apples which can theoretically assist the molecular breeding of disease-resistant apple crops.
AppleTranscriptomeAlternaria alternata apple pathotypeInfectionDefense response
Kai Liu、Zhaolin Liang、An Yang、Jiadi Yan、Peihua Cong、Xiaolei Han、Caixia Zhang
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Key Laboratory of Horticultural Crop Germplasm Utilization of the Ministry of Agriculture and Rural Affairs(MO A),Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China
国家自然科学基金现代农业产业技术体系建设项目Agricultural Science and Technology Innovation Program
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