首页|Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot

Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot

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Betalain has received increased attention because of its high nutritional value and crucial physiological functions.Based on the elucidation of its core biosynthetic pathway,betalain can be produced in additional plants by metabolic engineering.Synthesis of betalain in carrot(Daucus carota L.)can improve its nutritional quality and economic value by extracting betalain from the fleshy root,non-edible part,and processing residue of carrot.In this study,two different constructs,namely,pYB:mCD(AomelOS,BvCYP76AD1S,and BuDODA1S)and pYB:CDD(BuCY-P76AD1S,BvDODA1S,and MjcDOPA5GTS),were introduced into carrot for betanin synthesis by Agrobacterium-mediated transformation.Betanin can be synthetized in both transgenic calli,and pYB:mCD-transgenic callus can be used to produce betacyanin by suspension culture.However,pYB:mCD-transgenic seedlings can synthetize betanin only by tyrosine feeding.The pYB:CDD-transgenic lines can synthetize betanin in whole plants.The betanin content in fleshy root of pYB:CDD-transgenic carrot was(63.4±9)μg·g-1 fresh weight according to quantitative analysis.These betanin-producing carrot plant materials can be used to synthesize betanin for industrial application or consumption as dietary sources.

Daucus carota L.BetaninBiosynthesisMetabolic engineeringBiofortification

Bo Wang、Yahui Wang、Yuanjie Deng、Quanhong Yao、Aisheng Xiong

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State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China

Shanghai Key Laboratory of Agricultural Genetics and Breeding,Biotechnology Research Institute of Shanghai Academy of Agricultural Science,Shanghai 201106,China

国家自然科学基金Key Research and Development Projects of Ningxia Hui Autonomous RegionKey Project Fund of the Shanghai Municipal Committee of AgricultureKey Project Fund of the Shanghai Municipal Committee of AgricultureShanghai Academic Technology Research LeaderPriority Academic Program Development of Jiangsu Higher Education Institutions Project(PAPD)

320725632022BBF020082021-02-08-00-12-F00795Chuangzi 2018 1-519XD1432300

2024

10.1016/j.hpj.2023.05.012

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(3)
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