首页|Mutation in the Agrobacterium hisI gene enhances transient expression in pepper
Mutation in the Agrobacterium hisI gene enhances transient expression in pepper
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Agrobacterium-mediated plant transformation is widely used in plant genetic engineering.However,its efficiency is limited by plant im-munity against Agrobacterium.Chili pepper(Capsicum annuum L.)is an important vegetable that is recalcitrant to Agrobacterium-mediated transformation.In this work,Agrobacterium was found to induce a strong immune response in pepper,which might be the reason for T-DNA being difficult to express in pepper.An Agrobacterium mutant screen was conducted and a point mutation in the hisI gene was identified due to a weak immune response and enhanced transient expression mediated by this Agrobacterium mutant in pepper leaves.Further genetic analysis revealed that histidine biosynthesis deficiency caused by mutations in many genes of this pathway led to reduced pepper cell death,pre-sumably due to reduced bacterial growth.However,mutation analysis of threonine and tryptophan biosynthesis genes showed that the biosynthesis of different amino acids may play different roles in Agrobacterium growth and stimulating the pepper immune response.The possible application of Agrobacterium amino acid biosynthesis mutations in plant biology was discussed.
Dan Liu、Shengnan Zhao、Jubin Wang、Xi Zhang、Yingtian Deng、Feng Li
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National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan,Hubei 430070,China
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