首页|Editing of eIF(iso)4E.c confers resistance against Turnip mosaic virus in Brassica rapa

Editing of eIF(iso)4E.c confers resistance against Turnip mosaic virus in Brassica rapa

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Turnip mosaic virus(TuMV)constitutes one of the primary diseases affecting Brassica rapa,severely impacting its production and resulting in crop failures in various regions worldwide.Recent research has demonstrated the significance of plant translation initiation factors,specifically the eIF4E and eIF4G family genes,as essential recessive disease resistance genes.In our study,we conducted evolutionary and gene expression studies,leading us to identify eIF(iso)4E.c as a potential TuMV-resistant gene.Leveraging CRISPR/Cas9 technology,we obtained mutant B.rapa plants with edited eIF(iso)4E.c gene.We confirmed eIF(iso)4E.c confers resistance against TuMV through phenotypic observations and virus content evaluations.Furthermore,we employed ribosome profiling assays on eif(iso)4e.c mutant seedlings to unravel the translation landscape in response to TuMV.Interestingly,we observed a moderate correlation between the fold changes in gene expression at the transcriptional and translational levels(R2=0.729).Comparative analysis of ribosome profiling and RNA-seq data revealed that plant-pathogen interaction,and MAPK signaling pathway-plant pathways were involved in eIF(iso)4E.c-mediated TuMV resistance.Further analysis revealed that sequence features,coding sequence length,and normalized minimal free energy,influenced the translation efficiency of genes.Our study highlights that the loss of eIF(iso)4E.c can result in a highly intricate translation mechanism,acting synergistically with transcription to confer resistance against TuMV.

Brassica rapaeIF(iso)4E.cTuMVRibo-seq

Yujia Liu、Xiaoyun Xin、Peirong Li、Weihong Wang、Yangjun Yu、Xiuyun Zhao、Deshuang Zhang、Jiao Wang、Fenglan Zhang、Shujiang Zhang、Shuancang Yu、Tongbing Su

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State Key Laboratory of Vegetable Biobreeding,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China

National Engineering Research Center for Vegetables,Beijing Key Laboratory of Vegetable Germplasms Improvement,Key Laboratory of Biology and Genetics Improvement of Horticultural Crops(North China),Key Laboratory of Urban Agriculture(North)of Ministry of Agriculture,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Beijin

National Engineering Research Center for Vegetables,Beijing Key Laboratory of Vegetable Germplasms Improvement,Key Laboratory of Biology and Genetics Improvement of Horticultural Crops(North China),Key Laboratory of Urban Agriculture(North)of Ministry of Agriculture,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China

Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China

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Scientist Training Program of BAAFSInnovation and Capacity-Building Project of BAAFSCollaborative innovation program of the Beijing Vegetable Research CenterNational Natural Science Foundation of ChinaChina Agriculture Research System of MOF and MARA

JKZX202406KJCX20230221XTCX20230232072567CARS-A03

2024

10.1016/j.hpj.2024.05.001

园艺学报(英文版)

园艺学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.10(4)
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