首页|Identification of a hypersensitive response core peptide of HrpZ and its role in increasing grape downy mildew resistance
Identification of a hypersensitive response core peptide of HrpZ and its role in increasing grape downy mildew resistance
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
万方数据
Harpins play a key role in inducing disease resistance in crops,and identifying their core functional regions and establishing a system for their efficient expression would be very valuable.In this study,large amounts of soluble fusion proteins of harpin HrpZ and its subpeptides were obtained via the optimized induction conditions(28 ℃ with 0.5 mmol-L-1 IPTG for 6 h)in Escherichia coli BL21(DE3).Hypersensitive response(HR)assays demonstrated that the C-terminal 66 aa of HrpZ(HrpZ_C_2_2)elicited a strong HR in tobacco(Nicotiana benthamiana)and grape(Flame Seedless)leaves.Additionally,treatment with HrpZ,and particularly HrpZ_C_2_2,significantly reduced the disease incidence and severity index of field vine leaves and those inoculated with downy mildew.The determination of the physiological parameters indicated that HrpZ,and especially HrpZ_C_2_2,improved the photosynthesis-and chlorophyll fluorescence-related parameters,enhanced the activity of defense-related enzymes,including SOD,POD,CAT and PAL,and increased the H2O2 level.Collectively,we efficiently expressed a core peptide of HrpZ and elucidated its strong ability to elicit a HR and resistance to downy mildew.This research provides insight into understanding the structure and function of HrpZ and will advance the application of HrpZ_C_2_2 to increase the resistance of grapevine to downy mildew.
Zongbao Fan、Xueqiang Guan、Zhichang Zhang、Yushuai Sun、Fei Wang、Huiru Chi、Yuxin Yao
展开 >
State Key Laboratory of Crop Biology,Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production,College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China
Shandong Academy of Grape/Shandong Technology Innovation Center of Wine Grape and Wine,Jinan,Shandong 250100,China
Zhichang Agricultural Science and Technology Development Co.,Ltd.,Rizhao,Shandong 276511,China
万方数据
