HPLC法测定枳壳中6个二氢黄酮类成分的含量
Quantification of six flavonones in Aurantii Fructus by HPLC
林宗涛 1陈世忠 1王弘1
作者信息
摘要
目的:建立HPLC法测定枳壳药材中6个黄酮类成分圣草次苷、新圣草苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷的含量.方法:采用Inertsil ODS-4(4.6 mm×150 mm,5μm)色谱柱,流动相为乙腈-0.1％磷酸(18:82),流速1.0 mL·min-1,检测波长283 nm,柱温30℃.结果:圣草次苷、新圣草苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷6个二氢黄酮类化合物的质量分别在0.02 ～0.16、0.60 ～0.54、0.02 ～0.18、0.41 ～3.70、0.16 ～ 1.40、0.22～ 1.99 μg范围内与峰面积呈良好的线性关系,r分别为0.9995、0.9999、0.9999、0.9999、0.9999和0.9999;平均加样回收率(n=6)分别为100.8％、102.5％、97.8％、99.6％、98.0％、102.7％.结论:含量测定方法可作为中药枳壳的质量控制的有效方法.
Abstract
Objective: To establish an HPLC method for simultaneous determination of eriocitrin, neoeriocitrin, na-rirutin, naringin, hesperidin, neohesperidin content in Aurantii Fructus. Methods: HPLC separation was carried out with an Inertsil ODS -4 column(4. 6 mm × 150 mm,5 μm) using acetonitrile -0. 1% phosphoric acid( 18: 82) as mobile phase at a flow rate of 1.0 mL · min-1 . The detection wavelength was 283 nm and the column temperature was maintained at 30 ℃. Results: Good linear relationships were observed between the masses and the peak areas of eriocitrin, neoeriocitrin, narirutin, naringin, hesperidin, neohesperidin in the ranges of 0.02-0.16(r=0. 9995) , 0.60-0.54(r=0.9999),0.02-0.18(7-=0.9999),0.41 -3.70(r =0. 9999) ,0. 16 - 1. 40(r =0.9999) and 0. 22 - 1. 99(r = 0. 9999) μg, respectively; The average recovery rates ( n = 6 ) were 100. 8% , 102. 5% ,97. 8% , 99. 6% ,98.0% and 102. 7% Respectively. Conclusion:The established quantification method is applicable for the quality control of Aurantii Fructus.
关键词
枳壳药材/二氢黄酮/圣草次苷/新圣草苷/芸香柚皮苷/柚皮苷/橙皮苷/新橙皮苷/高效液相色谱法/含量测定Key words
Aurantii Fructus/flavonone/eriocitrin/neoeriocitrin/narirutin/naringin,hesperidin,neohesperidin/HPLC/assay引用本文复制引用
基金项目
国家科技重大专项-中药质量标准研究和信息化体系建设平台项目(2009ZX09308)
出版年
2013
NETL
NSTL
维普
万方数据