Determination of three saponins in Panax notoginseng by NIR
Objective:To establish an NIR (near-infrared)quantitative analysis model of three saponins (notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1) in Notoginseng Radix et Rhizoma,so as to quickly analyze the quality of medicinal materials.Methods:The contents of three saponins in 72 batches of Notoginseng Radix et Rhizoma were determined according to Chinese Pharmacopoeia (2015).Near infrared spectroscopy was collected,and standard normal variate (SNV)and the first derivative (db1)were used to pretreatment spectra.In addition,a notoginsenoside near-infrared (NIR) quantitative analysis model was established combining with partial least square method.Results:The established model of 67 batches of Notoginseng Radix et Rhizoma showed that the correlation coefficient was (r) =0.990 3,calibration set root mean square deviation was (RMSEC) =0.353 5,the prediction set root mean square deviation was (RMSEP) =0.458 0.Then,we validated the model applying five Panax notoginseng samples beyond the established model,and the forecast average recovery of validation set was 101.12％.Conclusion:The established model in this study is accurate enough to determine the content of three saponins in Panax notoginseng.It can be used in quality testing of Panax notoginseng.Near infrared spectroscopy is fast,simple and accurate,which provides a basis for quality control of Panax notoginseng.decoction Pieces.
NETL
NSTL
万方数据
维普
