Determination of 7 active components in volatile oil of Amomis Fructus
Objective:To establish a method for the simultaneous determination of α-pinene,camphene,myrcene,limonene,camphor,borneol,bornyl acetate in volatile oil by GC,so as to provide a reference for the quality control of Amomis Fructus.Methods:A DB-5 (30 m × 0.320 mm × 0.25 μ m) column was adopted with nitrogen as carrier gas and FID as detector.The column temperature was controlled by program.The inlets temperature was 230 ℃,and the FID detector temperature was 250 ℃.Bornyl acetate was used as the internal reference to get the relative correction factors (RCF) of borneol and camphor in ratios to the reference.The contents of three components in all batches of Amomis Fructus were calculated and the quantitative results were compared by t-test between QAMS and ES method.The other four componenets were determined by ES.Results:Seven compounds had good linear relationship,precision,stability and repeatability according to the requirements of the methodology determination.The linear range of α-pinene,camphene,myrcene,limonene,camphor,borneol and bornyl acetatec were 3.380-169.0 mg· mL-1 (r=0.999 8),13.90-696.0 mg· mL-1 (r=0.999 9),4.200-210.0 mg· mL-1 (r=0.999 9),18.20-908 mg· mL-1 (r=0.999 9),10.50-525.0 mg· mL-1 (r=0.999 9),9.58-479.0 mg· mL-1 (r=0.999 8) and 32.60-1.630 × 103 mg· mL-1 (r=-0.999 9).The average recoveries were 101.3％ (RSD=1.9％),101.5％ (RSD=2.6％),101.9％ (RSD=1.2％),101.3％ (RSD=2.3％),101.0％ (RSD=1.6％),101.7％ (RSD=1.3％) and 99.9％ (RSD=2.2％),respectively.The contents of α-pinene,camphene,myrcene,limonene,camphor,borneol and bornyl acetatec in volatile oil were ranged in 0.117 0-0.430 5,0.450 4-3.734,0.101 0-1.808,0.319 6-3.687,1.660-10.40,0.290 0-1.640 and 0.454 7-13.16 mg· g-1,respectively.Conclusion:The established method is simple,convenient and with good specificity,and could be used as a valid analytical method to evaluate the intrinsic quality of Amomi Fructus.
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NSTL
万方数据
维普
