Optimization of extracting process of bergenin from the roots of Ardisia crenata by response surface methodology
Objective:To optimize the technology for bergenin extraction from the roots of Ardisia crenata.Methods:Based on the single factor test,the three factors of extracting time,solid to liquid ratio,and extracting power were studied by the orthogonal test developed from Box-Behnken central composite test design.Meanwhile,the relative equation was established.Using the response surface method,the result was analyzed and the extracting process was optimized.Results:The optimized conditions were as follows:the extracting time was 41.21 min,solid to liquid ratio was 10.14∶1 (mL· g-1),the extrating power was 108.83 W,and the extraction rate was 2.233％.In order to facilitate the practice,the conditions were modified as follows:the extracting time was 42 min,solid to liquid ratio was 10 ∶ 1 (mL· g-1),and the extracting power was 109 W.The experimental results was verified with an average extraction rate of 2.318％,which was close to the model predicted value.Conclusion:The mathematical model established has high presision (P＜O.O1),which can be used to analyze and predict the extraction process of bergenin from Ardisia crenata.
NETL
NSTL
万方数据
维普
