钴60辐照对柴胡药材有效成分的影响研究
Effect of radiant sterilization on the contents of effective components of Radix Bupleuri
吕望 1徐放 2吴赛伟 1虞和永1
作者信息
- 1. 浙江大学医学院附属第四医院,义乌322000
- 2. 东北林业大学生命科学学院,哈尔滨150040;黑龙江中医药大学基础医学院,哈尔滨150040
- 折叠
摘要
目的:建立柴胡药材活性成分含量的HPLC测定方法,考察钴60射线辐照前后活性成分含量的变化.方法:采用C18色谱柱(4.6 mm×250 mm,5μm),流动相为0.1％磷酸水溶液-乙腈,梯度洗脱,流速1.0 mL·min-1,检测波长210 nm,柱温30℃.分别选择剂量为5、8、10 kGy对柴胡药材进行辐照,比较辐照前后活性成分含量的变化,并进行成组t检验考察其显著情况.结果:柴胡皂苷a、c、d质量浓度分别在0.020～0.806、0.022～0.893及0.025～1.003 mg· mL-1范围内有良好线性,平均加样回收率分别为99.4％、98.7％和99.8％;未经辐照的6批柴胡药材中柴胡皂苷a、c、d含量范围分别为3.023～3.245、1.034～1.341和5.023～5.298 mg·g-1;经10 kGy辐照后,上述3个成分的含量范围分别为2.975～3.226、0.823～0.978和4.963～5.136 mg· g-1;经过5、8、10 kGy辐照后,柴胡药材所含的成分柴胡皂苷a、c、d均有变化,经成组t-检验,在超过5 kGy后,柴胡皂苷c辐照前后含量变化显著(P＜0.01).结论:所建立的柴胡活性成分测定方法可作为柴胡质控方法.在辐照剂量不超过5 kGy时,各成分变化不显著,可为柴胡药材辐照灭菌手段提供参考.
Abstract
Objective:To establish an HPLC method for determination of effective ingredients in Radix Bupleuri,and to investigate the effect of 60Co-γ ray irradiation on the effective components.Methods:The C18 column (4.6 mm × 250 mm,5 μm) was adopted and the mobile phase consisted of 0.1％ acetic acid(A) and acetonitrile (B)with a gradient elution at the flow rate of 1.0 mL· min-1,the wavelength of UV detection was 210 nm,and the column temperature was 30 ℃.Radix Bupleuri was irradiated at the dose of 5,8 and 10 kGy.Then contents of the active ingredients before and after irradiation were compared by t-test.Results:The hnear range of saikosaponin a,saikosaponin c and saikosaponin d were 0.020-0.806 mg· mL-1,0.022-0.893 mg· mL-1 and 0.025-1.003 mg· mL-1,respectively,and the average recoveries were 99.4％,98.7％ and 99.7％,respectively.The content ranges of saikosaponin a,saikosaponin c and saikosaponin d in 6 non-irradiated Radix Bupleuri were 3.023-3.245 mg· g-1,1.034-1.341 mg· g-1 and 5.023-5.298 mg· g-1,respectively;while the content ranges of those in the samples irradiated at 10 kGy were 2.975-3.226 mg· g-1,0.823-0.978 mg· g-1 and 4.963-5.136 mg· g-1,respectively.After irradiation at 5,8 and 10 kGy,saikosaponin a,saikosaponin c and saikosaponin d in Radix Bupleuri changed.And significant difference was found between contents of saikosaponin c in samples irradiated at 5 kGy.Conclusion:The established method could be used in quality control of Radix Bupleuri.When the radiation was no more than 5 kGy,each active ingredient did not change significantly.The proposed method provided reference for sterilization of Radix Bupleuri.
关键词
柴胡/钴60/柴胡皂苷/含量变化/辐照灭菌/保鲜技术/安全监测/高效液相色谱Key words
Radix Bupleuri/60Co-γγ ray/saikosaponin/content variation/radiant sterilization/keeping fresh/preservation technology/HPLC引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据