气相色谱-静电场轨道阱质谱(gas chromatography-Orbitrap mass spectrometry,GC-Orbitrap/MS)作为新兴的高分辨气质联用技术,可实现挥发性成分的精确质量检测和高通量定性定量分析,具有灵敏度高,选择性强,线性动态范围宽等优点,能够很好地应对复杂基质中多种痕量物质分析带来的挑战,近年已开始应用于环境、工业、食品、制药、司法、临床等领域.本文对GC-Orbitrap/MS技术进行全面综述,阐述其基本原理及技术特点,介绍该技术在食品与药品分析研究中的应用进展,包括食品中农药残留检查、持久性有机物检测、香气物质分析和化学药品杂质分析与中药质量评价,并重点分析在超痕量成分测定和未知化合物鉴别方面的优势.最后,从高分辨率标准数据库的建立、可变电子电压技术的应用、样品前处理方式的选择开发与优化、二维GC-Orbitrap/MS串联等角度,提出GC-Orbitrap/MS未来发展方向并展望其应用前景.
Recent advances of gas chromatography-Orbitrap mass spectrometry in food and pharmaceutical analysis
Gas chromatography-Orbitrap mass spectrometry(GC-Orbitrap/MS),a developing gas chromatogra-phy-high resolution mass spectrometry approach,allows for high throughput qualitative and quantitative analysis of volatile and semi-volatile components.It has the advantages of high sensitivity,selectivity,and a wide linear dynamic range,which makes it well suitable for the analysis of a wide range of trace substances in complex matrices.In recent years,this technology has been applied in environmental science,industry,food analysis,pharmaceutical analysis,forensic science,clinical medicine and other fields.This paper presents the first review of GC-Orbitrap/MS,which not only describes the basic principles and technical characteristics,but also introduces the progress of the technique in food and pharmaceutical research.Applications in food analysis include the inspection of pesticide residues,detection of persistent organic compounds and analysis of flavor substances.In pharmaceutics,the analysis of chemical impurities and quality evaluation of traditional Chinese medicine are introduced.It is noteworthy that this technique is particularly advantageous for the identification of unknown compounds and the determination of ultra-trace components.Lastly but importantly,this review summarizes the challenges encountered in the current development of this technique,including the establishment of high-resolu-tion standard databases,the selection and optimization of sample pre-treatment method and the application of GC-Orbitrap/MS in the field of traditional traditional Chinese medicine.A few solutions are also proposed,such as the application of variable electron voltage technique,the combination of two-dimensional gas chromatography and electrostatic field Orbitrap mass spectrometry and the integrated analysis comprehensively using multiple scan modes.These strategies are aimed to provide more advanced and accurate solutions to food,pharmaceutical,and other relevant analysis.
gas chromatography-Orbitrap mass spectrometryhigh resolution mass spectrometryfoodpharma-ceuticalpesticide residuespersistent organic pollutantaroma substanceimpurity analysisquality evaluation of traditional Chinese medicinereview
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维普
