Objective:To prepare and investigate the content determination of Nocardia rubra cell wall skeleton(N-CWS)loading film.Methods:Taking chitosan and polyvinyl alcohol as film-forming materials,N-CWS film was prepared by solution casting method.The film was characterized by scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FTIR)and universal tensile testing machine.Determination of arabinose in N-CWS film was carried out by HPLC.The Kromasil C18column(150 mm ×4.6 mm,5 μm)was adopted,with the elution of 50 mmol·L-1 phosphate buffer(pH 7.0)-acetonitrile(80∶20,v/v).The flow rate was 1.0 mL·min-1,the detection wavelength was 254 nm,and the column temperature was 40 ℃.Results:When the mass ratio of chitosan to polyvinyl alcohol was 3∶2 and the mass ratio of membrane material to glycerol was 1∶2,the tensile modulus reached 2.47 mPa and the tensile strain at break was 136.67％.Scanning electron micros-copy showed that N-CWS was distributed asymmetrically on the bottom of the film.The linear relationship of D-arabinose in the prepared film was good in the range of 1.1-220 μg·mL-1,and the average recovery rate was 99.5％.The determination results of three batchs N-CWS films showed that the contents of D-arabinose in the films were(0.988±0.041)mg·g-1,(1.035±0.049)mg·g-1,(1.088±0.046)mg·g-1,respectively.Conclusion:The preparation process of N-CWS loading film is simple and feasible,and the content determination method is accurate and fast.This study can provide reference for the development of new dosage forms of N-CWS.
维普
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