红色诺卡氏菌细胞壁骨架药膜的制备与含量测定

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中文摘要：目的:制备红色诺卡氏菌细胞壁骨架(N-CWS)药膜并考察其含量测定方法.方法:以N-CWS为模型药物,壳聚糖、聚乙烯醇为成膜材料,通过溶液浇铸法制备N-CWS药膜;使用扫描电子显微镜(SEM)、红外光谱仪、万能试验机对药膜性能进行表征;采用柱前衍生化高效液相色谱法测定药膜中D-阿拉伯糖的含量,采用Kromasil C18(150 mm ×4.6 mm,5 µm)色谱柱,以50 mmol·L-1(pH 7.0)磷酸盐缓冲液-乙腈(80∶20,v/v)为流动相,流速1.0 mL·min-1,检测波长254 nm,柱温40℃.结果:当壳聚糖与聚乙烯醇质量比为3∶2,膜材与甘油质量比为1∶2时,断裂拉伸应变为136.67％,拉伸应力为2.47 mPa.扫描电镜观察到N-CWS主要分布在膜的底面,呈不对称分布;制备的药膜中D-阿拉伯糖在1.1～220μg· mL-1范围内线性关系良好,平均加样回收率为99.5％,测得3批次药膜中D-阿拉伯糖分别为(0.988± 0.041)mg·g-1、(1.035±0.049)mg·g-1、(1.088±0.046)mg·g-1.结论:N-CWS 药膜制备工艺简单易行,含量测定方法准确快速,本研究可为N-CWS的新剂型开发提供参考依据.

外文标题：Preparation and content determination of a Nocardia rubra cell wall skeleton film

外文摘要：Objective:To prepare and investigate the content determination of Nocardia rubra cell wall skeleton(N-CWS)loading film.Methods:Taking chitosan and polyvinyl alcohol as film-forming materials,N-CWS film was prepared by solution casting method.The film was characterized by scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FTIR)and universal tensile testing machine.Determination of arabinose in N-CWS film was carried out by HPLC.The Kromasil C18column(150 mm ×4.6 mm,5 μm)was adopted,with the elution of 50 mmol·L-1 phosphate buffer(pH 7.0)-acetonitrile(80∶20,v/v).The flow rate was 1.0 mL·min-1,the detection wavelength was 254 nm,and the column temperature was 40 ℃.Results:When the mass ratio of chitosan to polyvinyl alcohol was 3∶2 and the mass ratio of membrane material to glycerol was 1∶2,the tensile modulus reached 2.47 mPa and the tensile strain at break was 136.67％.Scanning electron micros-copy showed that N-CWS was distributed asymmetrically on the bottom of the film.The linear relationship of D-arabinose in the prepared film was good in the range of 1.1-220 μg·mL-1,and the average recovery rate was 99.5％.The determination results of three batchs N-CWS films showed that the contents of D-arabinose in the films were(0.988±0.041)mg·g-1,(1.035±0.049)mg·g-1,(1.088±0.046)mg·g-1,respectively.Conclusion:The preparation process of N-CWS loading film is simple and feasible,and the content determination method is accurate and fast.This study can provide reference for the development of new dosage forms of N-CWS.

外文关键词：

Nocardia rubra cell wall skeletonchitosanpolyvinyl alcoholfilmpre-column derivatizationcon-tent determinationD-arabinose

作者：

杨煌建、王娟、陈洲琴、程贤、严凌斌、林仙菊、张祝兰、连云阳

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作者单位：

福建省微生物研究所福建省新药(微生物)筛选重点实验室,福州 350007

关键词：

红色诺卡氏菌细胞壁骨架壳聚糖聚乙烯醇药膜柱前衍生化含量测定 D-阿拉伯糖

基金：

福建省属公益类研究所基本科研专项福建省属公益类研究所基本科研专项福州市科技创新平台项目

项目编号：

2021R10050072022R10050072021-P-25

出版年：

2024

DOI：

10.16155/j.0254-1793.2024.01.05

药物分析杂志

中国药学会

药物分析杂志

CSTPCD北大核心

影响因子：1.039

ISSN：0254-1793

年,卷(期)：2024.44(1)

参考文献量17