Colloidal gold immunochromatographic assay for rapid detection of herbicide prometryn in traditional Chinese medicine
Objective:To establish an immunochromatographic method suitable for the rapid detection of residues of triazine herbicide prometryn in Chinese medicine,based on self-made prometryn antigen and monoclonal anti-body.Methods:An antibody conjugate amount of 7 μg and dilution configuration of 0.1 mol·L-1 PBS with 1％OVA,0.1％Tween-80 were determined,and dry test strips with a T-line scribing concentration of 0.05 mg· mL-1 and a C-line scribing concentration of 0.5 mg·mL-1 were used.The suitable method was selected to im-prove the sensitivity of colloidal gold immunochromatography test strip,reduce the matrix effect of traditional Chi-nese medicine in different parts,and detect a large number of traditional Chinese medicine.Results:The detec-tion sensitivity of the test strip could reach 1 ng·mL-1,and the detection limit of the actual sample could reach 0.1 mg·kg-1.The established method was suitable for the detection of prometryn residues in 40 kinds of Chinese medicinal materials such as Citri Reticulatae Pericarpium,Taraxaci Herba,Notoginseng Radix et Rhizoma and-Lonicerae Japonicae Flos.Conclusion:This method has the advantages of rapid and accurately detection,simple pretreatment,simple operation and strong generalization,and can be used as an effective method for rapid screen-ing of prometryn residues in the field,so as to ensure the quality and safety of traditional Chinese medicine.At the same time,this study has a certain reference significance for the development of rapid detection of agricultural res-idues in many kinds of traditional Chinese medicine.
prometryncolloidal gold test striptraditional Chinese medicineherbicidesrapid detectionappli-cabilitymatrix effects
万方数据
维普
