CircRNAs are a large class of endogenous single-stranded RNAs that are different from other linear RNAs,which are produced by back-splicing and fusion of either exons,introns,or both exon-intron into cova-lently closed loops.They are widely expressed in highly differentiated eukaryotes,and are closely related to vari-ous development and metabolic disease processes of organisms.They are characterized by stable structure,resist-ant to RNA degradation,conservation,and tissue-specific expression,making them ideal biomarkers for diagno-sis and prognosis.Traditional methods such as Northern blotting,qRT-PCR and microarray analysis provide use-ful information,however,they are subject to their own shortcomings.Traditional methods are restricted in large-scale promotion in clinical trials.In recent years,in order to solve these problems,some new detection methods have emerged.In this article,we reviewed the relevant progress of all current circRNA detection methods,expounded their advantages and limitations,and discussed the challenges and future development directions.
circRNA detectionnew methodbiomarkersamplificationmerits and demerits
NETL
NSTL
万方数据
