Determination and characterization of Poriae Cutis multi-component content based on wavelength conversion RP-HPLC method
Objective:To comprehensively evaluate the quality of Poriae Cutis,and to establish a dual wavelength switching HPLC method for comparing the characteristic spectra of Poriae Cutis and studying the content of 11 triterpenoid components,to provide reference for the qualitative and quantitative research of Poriae Cutis.Methods:Agilent 5 HC-C18(2)column(250 mmx4.6 mm,5 μm)was adopted.Acetonitrile solution(con-tain 3％tetrahydrofuran)(A)and 0.1％formic acid aqueous solution(B)were used as the mobile phase with gradient elution at a flow rate of 1.0 mL·min-1.The column temperature was 30 ℃ and the injection volume was 20 μL.The detection wavelengths were 210 and 243 nm.Results:The feature profiles developed were effective in identifying the 18 shared peaks.RSD for precision,repeatability and stability(48 h)tests were all less than 3.72％(n=6).The 11 chemical components to be measured were well separated,with good linearity in the mass range examined(all r ≥ 0.999 6).The average recovery rate was 95.4％-105.5％,and the RSD was 1.0％-3.1％.The RSDs of precision,repeatability,and stability(48 h)tests were all less than or equal to 3.0％(n=6).The results of similarity analysis showed that most of the origins of Poriae Cutis were very similar to each other.The results of content determination showed that among the 11 triterpenoid constituents,poricoic acid A accounted for the highest percentage in all batches of Poriae Cutis.In addition,the content of five components,poricoic acid A,dehydrotrametenolic acid,poricoic acid B,dehydroeburicoic acid and trametenolic acid,fluctua-ted relatively more,while the other components fluctuated more gently.No significant geographic variation in sam-ples from different origins.Conclusion:A method for the determination of Poriae Cutis characteristics and multi-component content was established,which laid the foundation for quality control of Poriae Cutis.
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